Sample loss has been a persistent problem in cryo-EM, a high-tech method for creating 3D models of molecules that reveal their inner structures. It occurs during an essential step of imaging preparation, when a sample is blotted with a filter to remove excess liquid. Frequently, much of the sample transfers to the filter, leaving little to nothing behind on the imaging platform for the electron camera to capture.
Magnetic beads method dramatically improves cryo-EM’s imaging capabilities
