Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM has long relied on the complex rotation of one-dimensional stripe illumination at three angles, requiring nine exposures to reconstruct a uniform super-resolution image. This greatly hinders imaging speed and causes unnecessary photobleaching, limiting the available information flux in live-cell imaging.
Triangle structured illumination microscopy developed for sustained live-cell super-resolution imaging
